Sequencing and mutagenesis of genes from the erythromycin biosynthetic gene cluster of Saccharopolyspora erythraea that are involved in L-mycarose and D-desosamine production

Article Abstract:

Filamentous soil bacterium Saccharopolyspora erythraea produces macrolide antibiotic erythromycin A. To determine the nucleotide sequence of two segments of erythromycin biosynthetic gene cluster, a research study on the biosynthesis of erythromycin deoxysugars L-mycarose and d-desosamine was carried out. The genes were subjected to DNA sequence analysis. Findings showed that 2,3-dehydration and reduction to from the 2-deoxysugar, 5-epimerization to form the L-sugar, 3-C-methylation and 4-ketoreduction were required to convert NDP-4-keto-6-D-glucose to NDP-L-mycarose.

Bacteria, DNA binding proteins, Mutagenesis, DNA sequencers

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The loading domain of the erythromycin polyketide synthase is not essential for erythromycin biosynthesis in Saccharopolyspora erythraea

Article Abstract:

Mutant strains of Saccharopolyspora erythrea without the initial loading acyltransferase (AT-L) domain or without both the AT-L and acyl carrier protein domains, were constructed utilizing recombinant DNA technology. These strains were still capable of producing erythromycin though at lower levels than that produced by the wild-type strain. Moreover, the AT-L domain expressed as a monofunctional enzyme complemented the deletion of this domain from the polyketide synthase, resulting in enhanced levels of erythromycin production.

Microbial enzymes, Macrolide antibiotics, Macrolides

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Gene disruption and replacement in the rapamycin-producing Streptomyces hygroscopicus strain ATCC 29253

Article Abstract:

The biochemical properties of rapamycin biosynthesis genes was analyzed after the introduction of DNA into Streptomyces hygroscopicus with the temperate phiC31 phage. Rapamycin production was inhibited by the specific deletion of the polyketide synthase open reading frames that mediated rapamycin biosynthesis in Streptomyces hygroscopicus strain ATCC 29253. However, specific gene disruption of two other PKS regions had no effect on the production of rapamycin or nigericin.

Antibiotics, Genetic aspects, Microbiological synthesis

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