Several archael homologs of putative oligopeptide-binding proteins encoded by Thermotoga maritima bind sugars

Article Abstract:

The hyperthermophilic bacterium Thermotoga maritima has shared many genes through horizontal gene transfer and several of these encode putative oligopeptide ATP binding cassette (ABC) transporters. The hypothesis that these transporters actually transport sugars by measuring the substrate affinities of their encoded substrate binding proteins (SBPs) is tested and since these SBPs bind oligosaccharides with micromolar to nanomolar affinities, it is proposed that they are used primarily for oligosaccharide transport.

Genetic transformation, Binding proteins

---

Coregulation of beta-galactoside uptake and hydrolysis by the hyperthermophilic bacterium Thermatoga neapolitana

Article Abstract:

The characteristics of the beta-glycoside uptake system of the thermophilic anaerobic bacterium Thermotoga neapolitana were analyzed to determine the mechanisms that regulate microbial transport system. Thermotoga neapolitana cells grown in a galactose or lactose nutrient media accumulated beta-D-thiogalactopyranoside (TMG) in the presence of pyruvate. Furthermore, glucose-grown cells exhibited enhanced TMG accumulation due to the repressive effects of glucose on the beta-galactosidase transport system.

Biological transport, Active, Active biological transport, Microbial metabolism, Physiological regulation, Glucose

---

Identification and cultivation of anaeorobic, synthrophic long-chain fatty acid-degrading microbes from mesophilic and thermophilic methanogenic sludges

Article Abstract:

A study was conducted to investigate long-chain fatty acid (LCFA)-degrading anaerobic microbes by enrichment, isolation, and RNA-based stable isotope probing (SIP). The results suggest that microbes involved in the degradation of LCFAs under methanogenic conditions might not belong only to the family Syntrophomonadaceae, as most anaerobic LCFA-degrading microbes do, but might also be found in phylogenetically diverse bacterial groups.

Analysis, In situ hybridization, Anaerobiosis, Nucleic acids, Nucleic acid isolation

Similar abstracts:

• Abstracts: Allelic variation of polymorphic locus lytB, encoding a choline-binding protein, from Streptococci of the mitis group
• Abstracts: Novel hydrophobic surface binding protein, HsbA, produced by Aspergillus oryzae. Characterization of an inducible phenylserine aldolase from Pseudomonas putida 24-1
• Abstracts: Simultaneous display of multiple foreign peptides in the FliD capping and FliC filament proteins of the Escherichia coli flagellum
• Abstracts: Development and assessment of a real-time PCR assay for rapid and sensitive detection of a novel thermotolerant bacterium, lactobacillus thermotolerans, in chicken feces
• Abstracts: Nutrient amendments in soil DNA stable isotope probing experiments reduce the observed methanotroph diversity

This website is not affiliated with document authors or copyright owners. This page is provided for informational purposes only. Unintentional errors are possible.