Simultaneous detection of Pseudomonas fragi, P. lundensis, and P. putida from meat by use of a multiplex PCR assay targeting the carA gene
Article Abstract:
Species-specific primers and a multiplex PCR assay are developed for the simultaneous identification and differentiation of Pseudomonas fragi, Pseudomonas lundensis, and Pseudomonas putida, which is based on the coamplification of different portions of the small subunit of the carbomoyl phosphate synthase gene (carA). The carA multiplex PCR is used for detecting the presence of the three Pseudomonas species in meat and it is proved to be effective in showing their evolution during the storage of meat.
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 2007
User Contributions:
Comment about this article or add new information about this topic:
Biotyping of enterotoxigenic Staphylococcus aureus by enterotoxin gene cluster (egc) polymorphism and spa typing analyses
Article Abstract:
Thirty-five Staphylococcus aureus strains, including 10 reference strains and 25 strains recovered from clinical specimens and food samples, were analyzed by PCR restriction endonucleases analysis (REA) of the egc operum and spa typing. By an analysis of their nucleotide sequence identities, a new homogeneous cluster of a sei variant, called the sei variant, was detected in six strains.
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 2006
User Contributions:
Comment about this article or add new information about this topic:
Enterotoxigenic potential of Staphylococcus intermedius
Article Abstract:
Some strains of Staphylococcus intermedius contain the staphylococcal enterotoxin sec gene and could therefore produce enterotoxin. These strains were found in dogs, horses, and humans.
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 2001
User Contributions:
Comment about this article or add new information about this topic:
- Abstracts: Simultaneous biodetoxifiation of S, N, and O pollutants by engineering of a carbazole-degrading gene cassette in a recombinant biocatalyst
- Abstracts: Genetic diversity of viable, injured, and dead fecal bacteria assessed by fluorescence-activated cell sorting and 16S rRNA gene analysis
- Abstracts: Rapid, sensitive, and discriminating identification of Naegleria spp. by real-time PCR and melting-curve analysis
- Abstracts: Evaluation of the Staphylococcus aureus class C nonspecific acid phosphatase (sapS) as a reporter for gene expression and protein secretion in gram-negative and gram-positive bacteria