Soybean chromosome painting: a strategy for somatic cytogenetics
Article Abstract:
Fluorescent in situ hybridization, PCR-primed in situ labeling, and molecular probes have been developed and used to identify and evolve DNA sequences for cytological identification and physical mapping of soybean somatic chromosomes. Use of different cloned examples of each family allows selective labelling of unique subsets of soybean chromosomes.Double hybridization with biotin and digoxigenin determine the overlap in the chromosomes between different probes. It is essential to use different procedures for single-copy and highly repetitive DNA sequences.
Publication Name: The Journal of Heredity
Subject: Biological sciences
ISSN: 0022-1503
Year: 1996
User Contributions:
Comment about this article or add new information about this topic:
Genetic independence of the nodulation-response gene loci - Rj1, Rj2, and Rj4 - in soybean
Article Abstract:
Hybridization analyses designed to identify linkage or clustering of genes in soybeans were carried out to study the segregation patterns of three genes regulating nodulation in the plant. These are Rj1, Rj2 and Rj4. The dominant and recessive allels of each gene were assayed. The genes were then paired and the chi-square value of linkage for each pair determined. No evidence favoring linkage batween any pair was established.
Publication Name: The Journal of Heredity
Subject: Biological sciences
ISSN: 0022-1503
Year: 1993
User Contributions:
Comment about this article or add new information about this topic:
Soybean genetic map of RAPD markers assigned to an existing scaffold RFLP map
Article Abstract:
A linkage map of Glycine max was formed by anchoring random amplified polymorphic DNA markers to an existing restriction fragment length polymorphism map.
Publication Name: The Journal of Heredity
Subject: Biological sciences
ISSN: 0022-1503
Year: 2000
User Contributions:
Comment about this article or add new information about this topic:
- Abstracts: Protein diversity from alternative splicing: a challenge for bioinformatics and post-genome biology. Functional discovery via a compendium of expression profiles
- Abstracts: Integrated cytogenetic map of chromosome arm 4S of A. thaliana: structural organization of heterochromatic knob and centromere region
- Abstracts: The winged helix transcription factor Foxc1a is essential for somitogenesis in zebrafish. Control of her1 expression during zebrafish somitogenesis by a Delta-dependent oscillator and an independent wave-front activity
- Abstracts: Molecular mechanism of membrane protein integration into the endoplasmic reticulum. The Sec61p complex mediates the integration of a membrane protein by allowing lipid partitioning of the transmembrane domain
- Abstracts: Commensal interactions in a dual-species biofilm exposed to mixed organic compounds. Dual labeling with green fluorescent proteins for confocal microscopy