Spatial distribution and stability of the eight microbial species of the altered schaedler flora in the mouse gastrointestinal tract
Article Abstract:
The spatial distribution, interanimal variation, and persistence of bacteria in the most complex defined-flora model system to date, that is- mice colonized with the eight strains of the altered schaedler flora (ASF) is determined. A comparison of the relative distribution of the ASF strains in feces and the colon indicated large differences, suggesting that fecal bacterial levels may provide a poor approximation of colonial bacterial levels.
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 2004
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Evaluation of 23S rRNA PCR primers for use in Phylogenetic studies of bacterial diversity
Article Abstract:
A study was conducted to incorporates data from large-scale sequencing efforts to develop new and evaluate existing bacterium-specific 23S rRNA PCR amplification primers. A comparison of 23S and 16S rRNA gene clone libraries constructed from replicate water samples yielded gross similarities but also some important differences.
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 2006
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PCR-induced sequence artifacts and bias: Insights from comparison of two 16S rRNA clone libraries constructed from the same sample
Article Abstract:
The contribution of PCR artifacts to 16S rRNA gene sequence diversity from a complex bacterioplankton sample is estimated. The recommendations are provided for modification of amplification protocols and for reporting diversity estimates at 99% sequence similarity as a standard.
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 2005
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- Abstracts: Relative ability of orally administered Lactobacillus murinus to predominate and persist in the porcine gastrointestinal tract
- Abstracts: Movement of viruses between biomes. Distribution and diversity of archael ammonia monooxygenase genes associated with corals
- Abstracts: Telomere elongation is under the control of the RNAi-based mechanism in the Drosophila germline. Drosophila atm/telomere fusion is required for telomeric localization of HP1 and telomere position effect
- Abstracts: Survival of indicator and pathogenic bacteria in bovine feces on pasture. Evaluation of colilert-18 for detection of coliforms and Eschericha coli in subtropical freshwater
- Abstracts: Chaperone-mediated folding and maturation of the Penicillin acylase precursor in the cytoplasm of Escherichia coli