Specific oligonucleotide primers for detection of lecithinase-positive Bacillus spp. by PCR

Article Abstract:

A PCR-based assay helps identify Bacillus cereus in foods. Experimental studies help design three oligonucleotide primers within the sequence for cereolysin AB, a cytolytic determinant encoding hemolytic and lecithin-hydrolyzing activities of B. cereus. Examination of the specificity of these primers using a combined PCR-hybridization assay reveals the significant specificity of these primers for isolates of the B. cereus group. This combined assay helps detect B. cereus in artificially contaminated milk and the detection limit is 1 CFU ml of milk.

Food, Food microbiology, Bacteriology, Bacillus cereus

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Detection of Cryptosporidium parvum in raw milk by PCR and oligonucleotide probe hybridization

Article Abstract:

Combination of PCR amplification and oligonucleotide probe hybridization (assay A) helps in better evaluation of the Cryptosporidium parvum in raw milk than assay B, which is based on the Laxer et. al. primer set. Assay A is effective in the detection of all bovine Cryptosporidium isolates except C. muris. Assay B amplifies the Eimeria acervulina DNA and detects the Giardia intestinalis DNA. The sensitivity in 20 ml of milk is 1 to 10 oocysts. There is a lack of reproducibility of when only few oocysts are present in the milk.

Analysis, Polymerase chain reaction, Cryptosporidiosis, Milk, Dairy bacteriology

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Rapid and quantitative detection of hepatitis A virus from green onion and strawberry rinses by use of real-time reverse transcription-PCR

Article Abstract:

An immunomagnetic capture method and a meal-time reverse transcription-PCR assay were used to quantify hepatitis A virus (HAV) in green onion and strawberry rinses. The combined protocol detected as low as 0.5 PFU HAV in produce rinses and concentrated HAV levels up to 20-fold.

Science & research, Vegetables and melons, Other Vegetable (except Potato) and Melon Farming, Berry crops, Strawberries, Strawberry Farming, Green Onions, Genetic aspects, Hepatitis A virus, Hepatovirus, Diseases and pests

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