Splice site choice and splicing efficiency are positively influenced by pre-mRNA intramolecular base pairing in yeast
Article Abstract:
A study of the regulatory mechanisms for splice site selection and splice site partner selection during pre-mRNA splicing is presented. The study protocol involved the analysis of the splicing of transcripts containing chimeric introns or splice site duplications derived from two yeast genes. The results indicate that splicing efficiency and relative splice site strength are influenced by strong context effects. Base-pairing between two intronic regions close to the 5' splice site and branchpoint could account for these context effects.
Publication Name: Cell
Subject: Biological sciences
ISSN: 0092-8674
Year: 1993
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Mixed mechanisms in yeast pre-mRNA splicing?
Article Abstract:
Construction of a genetic screen for mutants of the UPR (unfolded protein response) pathway has unveiled interesting discoveries about yeast pre-mRNA splicing. A missense mutation was identified in the tRNA ligase gene. tRNA ligase encodes the protein that joins the two half molecule substrates to prevent degradation of these molecules. The tRNA ligase mutant allele had no effect on RNA levels and on tRNA splicing. However, activation of the UPR pathway in mutants inhibited splicing and caused rapid degradation of pre-mRNA.
Publication Name: Cell
Subject: Biological sciences
ISSN: 0092-8674
Year: 1996
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Cross-intron bridging interactions in the yeast commitment complex are conserved in mammals
Article Abstract:
The cross-intron protein to protein interactions in the commitment complexes of both yeast and mammals are established by using mutant yeast and bovine genes. Interactions between the newly identified commitment complex component Msl5p (also BBP for branchpoint bridging protein) and both Mud2p which resembles human U2AF65 and the U1 snRNP protein Prp40p indicated that there is an existing link between the introns.
Publication Name: Cell
Subject: Biological sciences
ISSN: 0092-8674
Year: 1997
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