Genes involved in sister chromatid separation are needed for B-type cyclin proteolysis in budding yeast
Article Abstract:
Three yeast genes, CDC16, CDC23 and CSE1, have been found to be involved in the proteolysis of cyclin CLB2, a B-type cyclin, during the G1 phase and anaphase of cell division. Proteolysis of other unstable proteins such as SW15 and CLB5 is not dependent on these genes. CLB2 proteolysis starts at the initiation of anaphase, but a fraction of CLB2 does not undergo proteolysis and remains stable till the end of anaphase. The function of CSE1 is not known, while CDC23 and CDC16 appear to be involved in sister chromatid separation during the transition from metaphase to anaphase, which may involve proteolysis, and in cyclin destruction during anaphase and G1.
Publication Name: Cell
Subject: Biological sciences
ISSN: 0092-8674
Year: 1995
User Contributions:
Comment about this article or add new information about this topic:
Cohesins: chromosomal proteins that prevent premature separation of sister chromatids
Article Abstract:
Chromatid cohesion among sister chromatids as studied to identify the proteins involved in the process using fluorescent in situ hybridization. Mutants that lose chromosome which are capable of separating sister chromatids without an anaphase promoting complex were isolated. Results show that proteins such as Smc1p, Smc3p, Scc1p and Scc2p are involved in sister chromatid cohesion. Separation of sister chromatids in the anaphase stage of mitotic division is believed to be the cause of loss of cohesion where Scc1p dissociates from chromatin and failure of chromatids to align in metaphase results to tumorigenesis.
Publication Name: Cell
Subject: Biological sciences
ISSN: 0092-8674
Year: 1997
User Contributions:
Comment about this article or add new information about this topic:
An ESP1/PDS1 complex regulates loss of sister chromatid cohesion at the metaphase to anaphase transition in yeast
Article Abstract:
The separation of sister chromatids between metaphase and anaphase is mediated by the dissociation of the cohesin protein Scc1p. The dissociation of Scc1p from the chromatids is made possible by the liberation of the ESP1 protein from the ESP1/PDS1 complex mediated by the anaphase promoting complex (APC). APCs promote the dissociation of sister chromatids by liberating ESP1 and not by degrading Scc1p.
Publication Name: Cell
Subject: Biological sciences
ISSN: 0092-8674
Year: 1998
User Contributions:
Comment about this article or add new information about this topic:
- Abstracts: A PHD finger protein involved in both the vernalization and photoperiod pathways in Arabidopsis. Polycomb-group proteins repress the floral activator AGL19 in the FLC-independent vernalization pathway
- Abstracts: Chitinase B from Serratia marcescens BJL200 is exported to the periplasm without processing. Comparative studies of chitinases A and B from Serratia marcescens
- Abstracts: Cis-effects of heterochromatin on heterochromatic and euchromatic gene activity in Drosophila melanogaster. Bari-1, a new transposon-like family in Drosophila melanogaster with a unique heterochromatic organization
- Abstracts: The genetical and environmental determination of phally polymorphism in the freshwater snail Bulinus truncatus
- Abstracts: Microbial degradation of an aliphatic polyester with a high melting point, poly(tetramethylene succinate). Polylactide degradation by an Amycolatopsis sp