System using tandem repeats of the cA peptidoglycan-binding domain from Lactococcus lactis for display of both N- and C-terminal fusions on cell surfaces of lactic acid bacteria
Article Abstract:
A system is established for displaying heterologous protein to the C terminus of the peptidoglycan-binding domain (cA domain) of a major autolysin from Lactococcus lactis (AcmA). The cell surface has shown heterologous protein on the C terminus of the cA domain by using AmyA as a model protein and an increase in binding capacity by using tandem repeats of the cA domain.
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 2008
User Contributions:
Comment about this article or add new information about this topic:
Display of alfa-amylase on the surface of Lactobacillus casei cells by use of the PgsA anchor protein, and production of lactic acid from starch
Article Abstract:
A study was made on the fusion of the N terminus of the target protein with the PgsA protein by constructing a novel starch-degrading strain of Lactobacillus casei. It was observed that the recombinant L. casei cells with cell surface display of AmyA helped in the efficient simultaneous saccharification and fermentation from soluble starch to lactic acid.
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 2006
User Contributions:
Comment about this article or add new information about this topic:
Functional analysis of the fructooligosaccharide utilization operon in Lactobacillus paracasei 1195
Article Abstract:
The cell fractionation experiments which show the fructooligosaccharide (FOS) hydrolysis activity present exclusively in the cell wall extract of Lactobacillus paracasei grown on FOS is reported.
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 2007
User Contributions:
Comment about this article or add new information about this topic:
- Abstracts: Intracellular accumulation of trehalose protects Lactococcus lactis from freeze-drying damage and bile toxicity and increases gastric acid resistance
- Abstracts: Characterization of the lacticin 481 operon: the Lactococcus lactis genes lctF, lctE, and lctG encode a putative ABC transporter involved in bacteriocin immunity
- Abstracts: Surveillance of bulk raw and commercially pasteurized cow's milk from approved Irish liquid-milk pasteurization plants to determine the incidence of Mycobacterium paratuberculosis
- Abstracts: Evaluation of the Duopath Legionella lateral flow assay for identification Legionella pneumophila and Legionella species culture isolates
- Abstracts: Characterization of the meta-cleavage compound hydrolase gene involved in degradation of the lignin-related biphenyl structure by Sphingomonas paucimobilis SYK-6