T7 RNA polymerase bypass of large gaps on the template strand reveals a critical role of the nontemplate strand in elongation
Article Abstract:
T7 RNA polymerase are able to transcribe DNA containing gaps from one to five bases in the template strand. Interestingly, it is possible to transcribe the broken template strands missing up to 24 bases, although at reduced efficiency. The transcripts thus obtained incorporated the full template sequence with the RNA deleted for the gapped region missing on the template strand. It can be thus inferred that the end of a downstream template strand can be associated with the polymerase and transcribed as a part of an intact polynucleotide chain by using the unpaired nontemplate strand.
Publication Name: Cell
Subject: Biological sciences
ISSN: 0092-8674
Year: 1995
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Promoter proximal sequences modulate RNA polymerase II elongation by a novel mechanism
Article Abstract:
The mouse beta-globin promoter has sequences that prevent arrest at the adenovirus major late (ML) arrest site. Sequences near the 5' end of the transcript and sequences immediately upstream of the arrest site were identified as responsible for readthrough of the ML arrest site downstream of the beta-globin promoter. It was shown that anti-arrest required that this region of RNA form base pairs with the nascent transcript upstream of the arrest site. Small antisense RNA or DNA oligonucleotides hybridizing upstream of the arrest site also prevented arrest when added in trans.
Publication Name: Cell
Subject: Biological sciences
ISSN: 0092-8674
Year: 1996
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Base-specific recognition of the nontemplate strand of promoter DNA by E. coli RNA polymerase
Article Abstract:
Base-specific interaction between defined segments of DNA and the sigma subunit of the RNA polymerase in Escherichia coli allows the polymerase to recognize its promoters. Such recognition, in turn, leads to exposure of the template strand for RNA synthesis and separation of base pairs. Experiments indicate that the base-specific recognition of the sigma70 holoenzyme involves nontemplate strand bases in the -10 promoter region.
Publication Name: Cell
Subject: Biological sciences
ISSN: 0092-8674
Year: 1996
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