Targets for pSAM2 integrase-mediated site-specific integration in the Mycobacterium smegmatis chromosome
Article Abstract:
A study on new integrative plasmids has been conducted to improve the stability and frequency of the integration of pSAM2-based plasmids present in the mycobacterium smegmatis chromosome. Plasmid constructions, isolation of chromosomal DNA and Southern blot hybridization were the methods used in the study. Plasmid pLAS4 was also subjected to electroporation into M. smegmatis mc2155. Findings revealed that the plasmid was integrated into the chromosome as a result of the IntpSAM2-mediated site-specific recombination.
Publication Name: Microbiology
Subject: Biological sciences
ISSN: 1350-0872
Year: 1997
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The rRNA operons of Mycobacterium smegmatis and Mycobacterium tuberculosis: comparison of promoter elements and of neighbouring upstream genes
Article Abstract:
The regions of the two operons of Mycobacterium smegmatis upstream of its putative antitermination element is cloned and sequenced. RNA extracted from both M. smegmatis and Mycobacterium tuberculosis is used to compare the promoter regions of the two species using primer extension. It is shown that the operons have similar sequences in the upstream regions, an indication that they are closely related. M. smegmatis has four promoter elements while M. tuberculosis has two.
Publication Name: Microbiology
Subject: Biological sciences
ISSN: 1350-0872
Year: 1996
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The immunogenicity of recombinant Mycobacterium smegmatis bearing BCG genes
Article Abstract:
Recombinant pathogenic vectors have been found to have a high survival rate in vivo than plasmid vectors. Recombinant strains of Mycobacterium smegmatis which contained the genes for inducing tuberculosis survived at higher rates in the spleen and peritoneum of mice as compared to mice who were exposed only to the vector control. In addition, mice developed a resistance to Mycobacterium tuberculosis when they were treated by heat-killed J3R in Freund's adjuvant.
Publication Name: Microbiology
Subject: Biological sciences
ISSN: 1350-0872
Year: 1995
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