Inhibitory effect of aflastatin A on melanin biosynthesis by Colletotrichum lagenarium

Article Abstract:

Results point out that low concentrations of aflastatin inhibits the syntheses of melanin, the intermediate scytalone, and the polyketide synthase. Inhibition of scytalone is prevented by its addition in the presence of aflastatin, suggesting that the late steps after the scytalone synthesis are not affected by aflastatin in Colletotrichum lagenarium.

Japan, Physiological aspects, Microbial metabolism, Polyketides, Aflatoxins

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Cloning and structural analysis of the melanin biosynthesis gene SCD1 encoding scytalone dehydratase in Colletotrichum lagenarium

Article Abstract:

The deduced amino acid sequence of the phytopathogenic Colletotrichum lagenarium SCD1 gene, encoding scytalone dehydratase required for melanin biosynthesis, is homologous to the scytalone dehydratase sequence of Cochilobolus miyabeanus. SCD1 is a structural gene, and exists as a single copy in the genome. The gene consists of an open reading frame with 188 codons and two introns. The C. lagenarium mutant 9201Y(Scd-), which is defective in scytalone dehydratase activity, is transformed to its wild phenotype by the isolated genomic SCD1.

Nucleotide sequence, Base sequence, Microbiological synthesis

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Temporal transcriptional pattern of three melanin biosynthesis genes, PKS1, SCD1, and THR1, in appressorium-differentiating and nondifferentiating conidia of Colletotrichum lagenarium

Article Abstract:

Appressorial melanization in Colletotrichum lagenarium involves both transcriptional and posttranscriptional regulation of the three melanin biosynthesis genes, PKS1, SCD1, and THR1. In conidia incubated at 24 degree celsius, transcripts of the three melanin biosynthesis genes start accumulating 1 to 2 hrs after the start of incubation, decreasing at 6 hrs. The transcriptional pattern in conidia incubated at 32 degree celsius is similar to that in conidia incubated at 24 degree celsius. In conidia incubated in 0.1% yeast extract solution, transcripts hardly accumulate.

Genetic aspects, Genetic transcription, Transcription (Genetics)

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