The acidic transcriptional activation domains of VP16 and p53 bind the cellular replication protein A and stimulate in vitro BPV-1 DNA replication
Article Abstract:
Protein-Protein interaction between the acidic activation domains of VP16 and p53 and the cellular replication protein A (RPA) and the interaction between GAL4 DNA-binding domain and its binding sites together constitute a stable nucleoprotein complex for the initiation of in vitro BPV-1 DNA replication. The role of GAL4-VP16 fusion protein as an enhancer of replication depends on the integrity of the VP16 activation domain. The enhance proteins GAL4-p53 and GAL4-VP 16 negate the repressive effect of the core histones as they bind to the DNA, and ensure the assembly of the replication machinery.
Publication Name: Cell
Subject: Biological sciences
ISSN: 0092-8674
Year: 1993
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Association of the origin recognition complex with heterochromatin and HP1 in higher eukaryotes
Article Abstract:
Eukaryotic DNA replication requires the origin recognition complex (ORC). A marked preferential association of Drosophila ORC2 with heterochromatin on interphase and mitotic chromosomes has been observed. The association required the chromaodomain and shadow domains of HP1, a hetero-chromatin-localized protein. The results suggest that ORC may be important in packaging chromosomal domains.
Publication Name: Cell
Subject: Biological sciences
ISSN: 0092-8674
Year: 1997
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Mitotic-exit control as an evolved complex system
Article Abstract:
The four particularly intriguing features of the mitotic-exit control system are focused on, and these features from the perspective of evolutionary design and complex system engineering are examined. The traditional top-down engineering requires that all system behavior can be determined by specific components and that the behavior of the whole is precisely the sum of the parts.
Publication Name: Cell
Subject: Biological sciences
ISSN: 0092-8674
Year: 2005
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