The alkene monooxygenase from Xanthobacter strain Py2 is closely related to aromatic monooxygenases and catalyzes aromatic monohydroxylation of benzene, toluene, and phenol
Article Abstract:
The alkene monooxygenase from Xanthobacter strain Py2 was found to be homologous to aromatic monooxygenases toluene, phenol and benzene. This was gleaned from an experiment in which the genes encoding the six polypeptide components of the alkene monooxygenase were located on a 4.9-kb fragment of chromosomal DNA that was previously cloned in cosmid pNY2. Aside from the close association between the alkene monooxygenase and the aromatic monooxygenases, the alkene monooxygenase catalyzes the aromatic monohydroxylation of benzene, phenol and toluene.
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 1999
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The soluble membrane monooxygenase gene cluster of the trichloroethylene-degrading methanotroph Methylocystis sp. strain M
Article Abstract:
Soluble methane monooxygenase (sMMO) genes from Methylocystis sp. strain M were isolated by utilizing heterologous DNA probes from Methylosinus trichosporium strain OB3b. The Methylocystis sp. strain M sMMO genes cluster such as mmoX and mmoC exhibited similar DNA sequence to Methylosinus trichosporum OB3b. The amino acid sequence of the alpha subunit protein of Methylocystis sp. strain M was also highly conserved and similar to the ribonucleotide reductase of Methylococcus capsulatus and Methylosinus trichosporium.
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 1997
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Construction and use of a nontoxigenic strain of Pseudomonas aeruginosa for the production of recombinant exotoxin A
Article Abstract:
A mutant Pseudomonas aeruginosa strain PA103 which contains an inactive toxA gene does not produce any endotoxin A (ETA) or recombinant ETA but if toxA alleles are cloned into it, large amounts of ETA are formed. The strain PA01 does not produce as much ETA as the strain PA103 even when it contains the PA103A toxA gene which might be because the strain PA01 cannot form the gene regB which increases the transcription of toxA.
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 1995
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