The alkyl tert-butyl ether intermediate 2-hydroxyisobutyrate is degraded via a novel cobalamin-dependent mutase pathway

Article Abstract:

The degradation pathway of 2-hydroxyisobutyrate (HIBA) was examined in the methyl tert-butyl (MTBE)-degrading beta-proteobacterium strain L108, a novel isolate capable of growing on fuel oxygenate ethers as the sole source of carbon and energy, as well as in two tert-butyl alcohol (TBA)-degrading strains, a mutant of L108, strain L10 and strain CIP I-2052. Use of the mutase reaction results in an exceptional nutritional demand for cobalt during growth on substrates possessing the tert-butyl moiety.

Analysis, Ethers, Ethers (Class of compounds), Decomposition (Chemistry), Microbial genetics, Chemical properties, Alkyl groups, Vitamin B12

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Formation of trans fatty acids is not involved in growth-linked membrane adaptation of Pseudomonas putida

Article Abstract:

A study of fatty acid compositions in growing and resulting cells of several strains of Pseudomonas putida (P8, NCTC 10936, and KT 2440) is presented with an focus on alterations of the saturation degree, cis-trans isomerization, and cyclopropane formation. It is also demonstrated that harvesting and sample preparation for analysis could provoke the enzyme-catalyzed formation of trans fatty acids.

Pseudomonas putida, Isomerization, Trans fatty acids

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