The ice nucleation gene from Pseudomonas syringae as a sensitive gene reporter for promoter analysis in Zymomonas mobilis

Article Abstract:

Examination of the expression of the ice nucleation gene inaZ from Pseudomonas syringae in Zymomonas mobilis under the control of three distinct promoters reveals the efficiency of the inaZ gene as a sensitive gene reporter for promoter analysis in Z. mobilis. Ice nucleation spectra indicate the presence of both warm and cold nuclei in the cell pellets, while only the cold nuclei are observed in the culture supernatant, indicating that the cold nucleus activity is extracellular. Determination of ice nucleation activity at -9 degree Celsius helps compare the strengths of these three promoters.

Analysis, Genetic aspects, Gene expression, Promoters (Genetics), Pseudomonas syringae

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Isolation and properties of mutants of Zymomonas mobilis deficient in sugar assmilation

Article Abstract:

A study was conducted on the use of D-cycloserine to enrich Zymomonas mobilis (Z. mobilis) mutants deficient in sugar assimilation. The wild-type strain Zm4 of Z. Mobilis was subcultured thrice on fructose or glucose medium to enable gluctose- or fructose-negative mutants to emerge. The phenotype pf the various mutants were observed to be similar, supporting the assumption that they all have mutations in the same gene.

Bacteria, Microbial mutation

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Kinetic and nuclear magnetic resonance studies of xylose metabolism by recombinant Zymomonas mobilis ZM4(pZB5)

Article Abstract:

Energy restrictions appear to limit the growth of recombinant Zymomonas mobilis on xylose media. This yeast has been genetically engineered to convert xylose to ethanol.

Sugar, Microbial growth

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