Nondisruptive detection of activity of catabolic promoters of Pseudomonas putida with an antigenic surface reporter system
Article Abstract:
A procedure based on the immunodetection of a reporter epitope expressed on the surface of bacterial cells is effective in monitoring the switching on of catabolic promoters in Pseudomonas putida cells. An antigenic sequence of a coronvirus is linked to the naphthalene degradation plasmid of P. putida. The transcription of the plasmid exposes the surface epitope to monoclonal antibodies against the antigen. A measurement of the fluorescence of whole cells treated with monoclonal antibodies gives an idea of the switching on and off processes of the promoters.
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 1996
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Transcription and transcript processing in the sdhCDAB-sucABCD operon of Escherichia coli
Article Abstract:
The effects of global regulators and regulator defects on the suc and lpd promoters of succinate dehydrogenase and 2-oxoglutarate dehydrogenase were examined to understand how the expression of sucAB genes was co-regulated with other genes in the sdhCAB-sucABCD cluster and with lpdA expression. Results show that suc genes were transcribed and regulated from the sdh promoter and that ArcA influenced the co-expression of the sdh, suc and lpd genes. Moreover, it was also observed that the operon primarily transcribed from the sdh promoter.
Publication Name: Microbiology
Subject: Biological sciences
ISSN: 1350-0872
Year: 1998
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Modulation of gene expression through chromosomal positioning in Escherichia coli
Article Abstract:
A collection of hybrid mini-Tn5 transposons bearing lacZ fusions to the Psal promoter were analyzed to determine the variations in expressions of the nah genes of the NAH7 plasmid of Pseudomonas putida when placed in different chromosomal locations in escherichia coli. Results reveal that location modulates operative catabolic gene expression by virtue of the distance from the chromosomal replication origin. It was also found that chromosomal location affects the survival of clonal populations under antibiotic selective pressure.
Publication Name: Microbiology
Subject: Biological sciences
ISSN: 1350-0872
Year: 1997
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