The lipid A biosynthesis deficiency of the Escherichia coli antibiotic-supersensitive mutant LH530 is suppressed by a novel locus, ORF195

Article Abstract:

Antibiotic susceptibility determination and phage sensitivity testing were done to characterize a new Escherichia coli mutant designated LH530. The mutant was found to be supersensitive to several unrelated hydrophobic and large hydrophilic antibiotics. At 37 degrees Centigrade, LH530 was observed to grow poorly and not at all at 42 degree Centigrade. LH530 was also found to synthesize lipid A although the amounts produced are less than that of its parent strain. Experimental evidences indicated that such reduced lipid A biosynthesis was due to the inhibitory activity of the gene ORF195 found at 76 min on the E. coli chromosome.

Usage, Escherichia coli, Microbial sensitivity tests

---

Polyethyleneimine is an effective permeabilizer of Gram-negative bacteria

Article Abstract:

A research on the effect of Polyethyleimine (PEI) on various Gram-negative bacteria was conducted by concentrating on the phenomena identified with the permeability of the bacteria's outer membrane. (OM) Bacterial strain and permeability and bacteriolysis assays were the primary methodology used in the research. Findings showed that PEI makes gram-negative bacteria penetrable to hydrophobic investigations, such as antibiotics and NPN. However, it was also gleaned from the study that PEI did not have the capability to constrain the development of E. coli, P. aeruginosa, and other bacteria.

Gram-negative bacteria, Microbiological research, Permeability

---

Cellular fatty acyl and alkenyl residues in Megasphaera and Pectinatus species: contrasting profiles and detection of beer spoilage

Article Abstract:

The bacterial species commonly found in beer spoilage are characterized by the type of fatty acyl chains and alkenyl residues in their cellular composition. Megasphaera cerevisieae contained fatty acids that were similar to those found in M. elsdenii as well as alkenyl chains. Meanwhile, Pectinatus species yielded more odd numbered fatty acid chains, particularly 3-hydroxy fatty acids. These fatty acids may be used to detect the extent of spoilage in beer.

Physiological aspects, Beer, Fatty acids, Fatty acid synthesis

Similar abstracts:

• Abstracts: Colonization, persistence, and tissue tropism of Escherichia coli 026 in conventionally reared weaned lambs. Novel method for rapid assessment of antibiotic resistance in Escherichia coli isolates from environmental water by use of a modified chromogenic agar
• Abstracts: Inhibition of Escherichia coli biofilm formation by self-assembled monolayers of functional alkanethiols on gold
• Abstracts: Biotic and abiotic factors influencing in vitro growth of Escherichia coli O157:H7 in ruminant digestive contents. part 2
• Abstracts: Blimp-1 is an essential component of the genetic program controlling development of the pectoral limb bud. A germline GFP transgenic axolotl and its use to track cell fate: dual origin of the fin mesenchyme during development and the fate of blood cells during regeneration
• Abstracts: The signal transducer encoded by ampG is essential for induction of chromosomal AmpC beta-lactamase in Escherichia coli by beta-lactam antibiotics and 'unspecific' inducers

This website is not affiliated with document authors or copyright owners. This page is provided for informational purposes only. Unintentional errors are possible.