The yeast tRNA splicing endonuclease: A tetrameric enzyme with two active site subunits homologous to the archaeal tRNA endonucleases
Article Abstract:
Production of mature tRNA in organisms from all major phyla needs splicing of tRNA precursors. Through the splicing endonuclease of tRNA in yeast, the splice sites in pre-tRNA can be identified and split. Four protein subunits of endonuclease's gene encoding were cloned where every gene was found to be very essential. Based on the results of the experiment, the archaeal tRNA splicing endonuclease has two independent active sites for cleavage of 5' and 3' splice sites. Thus, the ancient origin for the tRNA splicing reaction was suggested by the presence of endonuclease in both Eucarya and Archaea.
Publication Name: Cell
Subject: Biological sciences
ISSN: 0092-8674
Year: 1997
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Depression of Her-2 uORF is mediated by a novel post-transcriptional control mechanism in cancer cells
Article Abstract:
Transcripts harboring 5' upstream open reading frames (uORFs) are often found in genes controlling cell growth including receptors, oncogenes or growth factors and uORFs can modulate translation or RNA stability and mediate inefficient translation of these potent proteins under normal conditions. In dysregulated cancer cells, where the gene product, for example Her-2 receptor, is overexpressed, post-transcriptional processes must exist that serve to override the inhibitory effects of uORFs.
Publication Name: Genes & Development
Subject: Biological sciences
ISSN: 0890-9369
Year: 2006
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Identification of a human endonuclease complex reveals a link between tRNA splicing and pre-mRNA 3' end formation
Article Abstract:
A study of the identification and characterization of the human tRNA splicing endonuclease, a fundamental process required for cell growth and division along with an alternatively spliced isoform of SEN2, which is part of the RNA endonuclease activity is presented. The findings show that human endonuclease complexes are associated with pre-mRNA 3' end processing factors, and pre-tRNA splicing suggests that endonuclease subunits function in multiple RNA-processing events.
Publication Name: Cell
Subject: Biological sciences
ISSN: 0092-8674
Year: 2004
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