Toluene elicits a carbon starvation response in Pseudomonas putida mt-2 containing the TOL plasmid pWW0

Article Abstract:

The effects of toluene on Pseudomonas putida mt-2(pWW0) strain were analyzed to determine the biochemical characteristics of the microbe's stress response to carbon starvation. Protein synthesis was inhibited in Pseudomonas putida mt-2(pWW0) strain that was cultured in a medium in which toluene was the sole source of carbon. Toluene also elicited carbon starvation response in Pseudomonas putida which produced 26 new proteins during starvation. Furthermore, carbon supplementation of toluene-exposed cells rapidly suppressed the production of these proteins.

Research, Microbial metabolism, Microbiological synthesis, Heat shock proteins, Toluene

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Integration of signals through Crc and PtsN in catabolite repression of Pseudomonas putida TOL plasmid pWW0

Article Abstract:

The global regulators involved in catabolite repression of the TOL plasmid promoters is identified and the o-xylene induction of TOL catabolic promoters in batch cultures in the presence and in the absence of one percent yeast extract (YE) are analyzed. It is found that in the early step of the regulatory process, catabolite repression control (Crc) acts by sensing the presence of YE.

Analysis, Nucleic acids, Hybridization, Nucleic acid isolation

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Propane and n-butane oxidation by Pseudomonas putida GPo1

Article Abstract:

Propane and n-butane inhibit methyl tertiary butyl ether oxidation by n-alkane-grown Pseudomonas putida strain. It was demonstrated that alkane hydroxylase activity was induced by both the gases and the same enzyme system used for the oxidation of n-octane was used for oxidation of both the gases.

Natural Gas Liquid Extraction, Natural gas liquids, Normal Butane, Butane-Propane Mix, Observations, Oxidation-reduction reaction, Oxidation-reduction reactions, Butane, Propane, Chemical properties

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