Towards the phylogeny of APS reductases and sirohaem sulfite reductases in sulfate-reducing and sulfur-oxidizing prokaryotes
Article Abstract:
A study was conducted on the cloned and sequenced genes of the adenosine-5'-phosphosulfate reductase, aprBA, and sirohaem sulfite reductase, dsrAB, from the sulfur-oxidizing phototrophic bacterium Chromatium vinosum strain D. The evolutionary distance between the enzymes from Chromatium vinosum and Desulfovibrio vulagaris and between those from Archaeoglobus fulgidus and Desulfovibrio vulagaris was determined by phylogenetic analyses. Results have shown that apr genes from organisms of different phylogenetic positions can be identified by using the aprA gene as a DNA probe.
Publication Name: Microbiology
Subject: Biological sciences
ISSN: 1350-0872
Year: 1997
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A dissimilatory sirohaem-sulfite-reductase-type protein from the hyperthermophilic archaeon Pyrobaculum islandicum
Article Abstract:
A sulfite-reductase-type protein was purified from the hyperthermophilic crenarchaeote Pyrobaculum islandicum grown chemoorganoheterotropically with thiosulfate as terminal electron acceptor. Similarly with dissimilatory sulfite reductases, the protein has an alpha-2-beta-2 structure and has high-spin siroheme, non-heme iron and acid-labile sulfide. The oxidized protein shows absorption maxima at 280, 392, 578 and 710 nm with shoulders at 430 and 610 nm. It has an unusual isoelectric point of 8.4.
Publication Name: Microbiology
Subject: Biological sciences
ISSN: 1350-0872
Year: 1998
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Sirohaem sulfite reductase and other proteins encoded by genes at the dsr locus of Chromatium vinosum are involved in the oxidation of intracellular sulfur
Article Abstract:
The in vivo role of 'reverse' sirohaem sulfite reductase was studied by determining the sequence of the dsr gene region of the phototrophic sulfur bacterium Chromatium vinosum D. Hybridization tests revealed that reduced sulfur compounds increased the expression of dsr genes and that photoorganoheterotrophic growth was not affected.A new sulfur oxidation model for the C. vinosum D bacterium can be developed from the findings.
Publication Name: Microbiology
Subject: Biological sciences
ISSN: 1350-0872
Year: 1998
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