Toxic effects on bacterial metabolism of the redox dye 5-cyano-2,3-ditolyl tetrazolium chloride
Article Abstract:
The monotetrazolium redox dye 5-cyano-2,3-ditolyl tetrazolium chloride (CTC), used to stain actively respiring bacteria, adversely affects bacterial growth and respiration. Samples treated with CTC have lower heterotrophic bacterial plate counts, bacterial production, and 14C glucose incorporation as compared to untreated control samples. Luminescence in a Microtox bioassay also demonstrates the toxicity of CTC. This implies that the CTC technique may provide wrong results while determining bacterial metabolic processes.
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 1996
User Contributions:
Comment about this article or add new information about this topic:
Methanogenic transformation of methylfurfural compounds to furfural
Article Abstract:
The methanogenic bacterium Methanococcus strain B uses the 5- and 2-methylfurfural compounds as growth substrate to convert them stoichiometrically into furfural. The metabolic transformation uses methyl group as a carbon source and carries out demethylation. This metabolic conversion finds application in anaerobic treatment of methylfurfural containing waste waters. Methylfurfural acts as an electron donor for methanogenesis.
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 1996
User Contributions:
Comment about this article or add new information about this topic:
Evidence for isotopic exchange during metabolism of stable-isotope-labeled formate in a methanogenic sediment
Article Abstract:
The stable-isotope-labeled and nonlabeled formate in freshwater methanogenic sediment is removed at the rate of 240 + or - 15 and 400 + or - 40 micro moles per hour, respectively. The removal of deuterium and 13C-labeled formate occurs with a simultaneous formation of the nonlabeled formate. The isotopic exchange due to the activity of dehydrogenase is responsible for the formation of nonlabeled formate.
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 1996
User Contributions:
Comment about this article or add new information about this topic:
- Abstracts: Effect of low temperatures on growth, structure, and metabolism of Campylobacter coli SP10. Enteric viruses of humans and animals in aquatic environments: Health risks, detection, and potential water quality assessment tools
- Abstracts: Differential susceptibility of aeromonads and coliforms to cefsulodin. A selective and differential medium for Vibrio harveyi
- Abstracts: Purification and characterization of two xylanases from alkalophilic Cephalosporium sp. strain RYM-202. Glycine betaine: reserve form of choline in Penicillium fellutanum in low-sulfate medium