Transcriptional control of ADH genes in the xylose-fermenting yeast Pichia stipitis

Article Abstract:

A study has been conducted to monitor changes brought about by gene expression processes of encoding group I alcohol dehydrogenase (PsADH1) in the yeast Pichia stipitis. It has been discovered that PsADH1 encodes a functional ADH protein inducible by oxygen limitation on xylose or glucose. Other conditions that induce alcohol dehydrogenase were found to induce PsADH1 transcriptions. The regulation of various levels of PsADH1 transcriptions in low oxygen environments was found to be extremely complex and any change would be difficult to monitor.

Analysis, Oxygen, Oxygen transport, Genetic regulation, Genetic transcription, Transcription (Genetics), Microbiological research

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Cloning and characterization of two pyruvate decarboxylase genes from Pichia stipitis CBS 6054

Article Abstract:

A study was conducted to determine the number and nature of the pyruvate decarboxylase (PDC) genes in Pichia stipitis. Two genes were cloned and sequenced from P. stipitis CBS 6054 to better characterize PDC expression and regulation. Results revealed that P. stipitis PDC1 was widely different from other yeast PDC genes and that the two P. stipitis genes have also diverged considerably from one another. Also, fermentative and PDC activities are enhanced with diminished oxygen instead of in response to fermentable sugars.

Genetic aspects, Enzymes, Enzyme structure-activity relationships, Decarboxylases

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Pichia stipitis genes for alcohol dehydrogenase with fermentative and respiratory functions

Article Abstract:

Research was conducted to isolate and characterize the alcohol dehydrogenase (ADH) genes essential for ethanol production in Pichia stipitis. Two distinct genes for ADH in P stipitis were identified, namely, PsADH1 and PsADH2. Gene disruption studies indicate that PsADH1 plays an important role in xylose fermentation since PsAH1 disruption leas to a lower growth rate and profoundly greater accumulation of xylitol.

Identification and classification, Bacterial genetics, Alcohol dehydrogenase, Alcohol dehydrogenases, Genetic code

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