Transcriptional regulation of the pdt gene cluster of Pseudomonas stutzeri KC involves an AraC/XylS family transcriptional activator (PdtC) and the cognate siderophore pyridine-2,6-bis(thiocarboxylic acid)
Article Abstract:
The characterization of a gene found in the pdt gene cluster of Pseudomonas stutzeri KC, predicted to have a regulatory role, is characterized in order to study the molecular mechanisms dictating production of the siderophore and dechlorination agent pyridine-2,6-bis(thiocarboxylic acid) (PDTC). Tests using the promoter of the PDTC gene in transcriptional reporter fusions pointed to siderophore-dependent negative autoregulation similar to that seen with other AraC-type regulators of siderphore biosynthesis and utilization genes.
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 2006
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Physiological factors affecting carbon tetrachloride dehalogenation by the denitrifying bacterium Pseudomonas sp. strain KC
Article Abstract:
The conditions for carbon tetrachloride (CT) transformation by Pseudomonas sp. strain KC were studied. Several carbon sources allowed CT transformation. It was shown that CT transformation was affected even by micromolar concentrations of soluble iron(II), iron(III), cobalt(II) and insoluble iron(III) oxyhydroxide. The addition of iron decreased CT removal by KC under aerobic and anaerobic conditions. Optimal conditions were used to search for CT transformation activity in denitrifying enrichments from aquifer samples.
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 1993
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Effects of medium and trace metals on kinetics of carbon tetrachloride transfromation by Pseudomonas sp. strain KC
Article Abstract:
Pseudomonas sp. strain KC is an aquifer-derived organism capable of transforming carbon tetrachloride (CT) to carbon dioxide and other nonvolatile products. The kinetics of CT transformation by strain KC was investigated. The results showed that CT transformation is a complex process that is linked to the iron-scavenging function of the cell and requires copper and a reducing agent. CT degradation by KC could be enhanced by selecting iron-rich ground waters or soil slurries and adjusting the environmental pH to 8.2.
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 1993
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