Transport of EDTA into cells of the EDTA-degrading bacterial strain DSM 9103

Article Abstract:

The transport of the complexing agent EDTA into whole cells of the EDTA-degrading bacterial strain DSM 9103 has been investigated. EDTA uptake was shown to be dependent on speciation, with free EDTA and metal-EDTA compounds having low stability constants readily taken up. CaEDTA transport was reduced by several uncouplers as well as an inhibitor of ATPase and the ionophore nigericin. On the other hand, valinomycin had insignificant effect on EDTA uptake, suggesting that the process may be induced by the proton gradient.

Biological transport, EDTA, Ethylenediaminetetraacetic acid, Bacterial motility, Complex compounds, Coordination compounds

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Molecular mechanisms of genetic adaptation to xenobiotic compounds

Article Abstract:

Microorganisms from various areas of the planet had been found to have developed evolutionary changes in the catabolism of manmade compounds. The influence of the amount of xenobiotic compounds introduced by man into the environment on the evolutionary process remains unanswered. However, it was known that gene transfer, mutational drift, and genetic recombination and transposition were the mechanisms that spurred the enviromental adaption.

Evaluation, Evolution (Biology), Microorganisms, Adaptation (Biology), Evolutionary adaptation, Xenobiotics

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Low-frequency horizontal transfer of an element containing the chlorocatechol degradation genes from Pseudomonas sp. strain B13 to Pseudomonas putida F1 and to indigenous bacteria in laboratory-scale activated-sludge microcosms

Article Abstract:

A study was conducted to examine low-frequency horizontal transfers of chlorocatechol degradative genes from Pseudomonas sp. strain B13 in activated-sludge microcosms. Matings were carried out with cultures pre-grown on Luria-Bertani medium that was washed and resuspended in Z3 mineral medium. The microcosms were characterized by glass reactors and operated at a volume of about 05. liter of culture medium.

Genetic aspects, Bacterial genetics, Pseudomonas putida

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