Two types of bacterial alginate lyases
Article Abstract:
Extracellular alginate lyases from Vibrio harveyi sp. AL-128 and V. alginolyticus ATCC 17749 were purified and characterized. The AL-128 enzyme was characterized as a poly(1,4-alpha-l-guluronide) lyase with a molecular weight of 57,000. Optimal pH is 7.8 and its isoelectric point is 4.3. Sodium was the most potent effector, with maximal activity at concentrations of 0.3 to 1.0 M sodium chloride. The ATCC 17749 enzyme is a poly(1,4-beta-d-mannuronide) lyase with a molecular weight of 47,000. Its optimal pH is 8.2 and the isoelectric point is 4.6. Optimal activity of the enzyme was seen with calcium chloride levels of 5 to 10 millimoles.
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 1992
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Bdellovibrios in Callinectus sapidus, the blue crab
Article Abstract:
Examination of blue crabs obtained from Chesapeak Bay reveal the presence of bdellovibrios specifically in gill tissues. Bdellovibrios are bacterial predators routinely present in soil and aquatic environments but rarely in animals. Colonization bythis organism of only the blue crab gills suggests that conditions prevailing in such location are critical to their survival. Epibiota samples from the ventral shell of crabs were also shown to harbor these organisms. However, thisassociation is non-specific as bdellovibrios can also be isolated from other surfaces in the aquatic environment.
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 1992
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Effects of temperature, salinity, and substrate on the colonization of surfaces in situ by aquatic bdellovibrios
Article Abstract:
The aquatic bacteria bdellovibrios prefer to use surfaces rather than water as habitat due to a rich source of prey bacteria on surface biofilms. The number of bdellovibrios and culturable bacteria on oyster shells are larger than those on glass and polystyrene substrates. Bdellovibrios are present on surfaces over a wide range of temperature and salinity on all substrates. The number of bdellovibrios from oyster shell and water samples is more at salinities greater than 11% as compared to those at lower-salinity environments.
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 1997
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