Use of a sentinel system for field measurements of Cryptosporidium parvum oocyst inactivation in soil and animal waste
Article Abstract:
A two-year study of the survival kinetics of oocysts in field settings on three watershed farms in New York, NY, is reported. Wastes from neonatal calves in these farms were being managed as part of dairy operations. A small-volume sentinel system was used to evaluate the effects of environmental stresses on survival of Cryptosporidium parvum oocysts in soil and animal wastes. Results suggested that manure piles containing C paevum oocysts can hasten inactivation and thus reduce the numbers of infective oocysts before wastes are spread in the soil environment.
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 1999
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Cytological effects of cellulases in the parasitism of Phytophthora parasitica by Pythium oligandrum
Article Abstract:
Research describes the in vitro interaction between Phythium oligandrum and Phytophthora parasitica at ultrastructural and cytochemical levels. Results indicate that the former affects the growth of the latter principally by attacking cellulose of the plasma membrane leading to cytoplasmic disorganization. Incorporating the observations into a biological control model is presented.
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 2000
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Quantitative flow cytometric evaluation of maximal Cryptosporidium parvum oocyst infectivity in a neonate mouse model
Article Abstract:
Research was conducted to determine the viability and infectivity of the Cryptosporidium parvum oocysts using a suckling mouse model. Results indicate that the infectivity is dependent on the dosage of the oocysts and ranged from 70 to 100%.
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 2000
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- Abstracts: Development of a PCR protocol for sensitive detection of Cryptosporidium oocysts in water samples. Simplified method for recovery and PCR detection of Cryptosporidium DNA from bovine feces
- Abstracts: An in vitro method for detecting infectious Cryptosporidium oocysts with cell culture. Evaluation of immunomagnetic separation for recovery of Cryptosporidium parvum and Giardia duodenalis from high-iron matrices
- Abstracts: Method detection limits of PCR and immunofluorescence assay for Cryptosporidium parvum in soil. Characterization of Cryptosporidium parvum by mastrix-assisted laser desorption ionization-time of flight mass spectrometry
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