Use of an oligonucleotide probe to detect Vibrio parahaemolyticus in artificially contaminated oysters
Article Abstract:
An oligonucleotide probe, designated VP5 and derived from the hemolysin gene, was developed to detect for the presence of Vibrio parahaemolyticus in shellfish. Tests on artificially contaminated oysters showed that the probe could detect V. parahaemolytucus at levels of about 10(super 6) colony forming units. DNA hybridization tests confirmed the probe's specificity to V. parahaemolyticus, although it did not hybridize to DNA of five V. parahaemolyticus isolates. These results demonstrate that VP5 may be used to directly identify V. parahaemolyticus in food samples.
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 1992
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Detection of hepatitis A virus RNA in oyster meat
Article Abstract:
Low concentrations of viruses in shellfish can be detected through nucleic acid amplification by PCR (RT-PCR). Hepatitis A virus (HAV) was detected in oyster meat by reverse transcription-PCR. A technique for identifying HAV RNA by RT-PCR of total RNA extracted from oyster meat contaminated by adsorption, bioaccumulation or injection, was developed. Using dot blot hybridization detection of amplicons from the RT-PCR, fast screening of a huge number of samples is possible.
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 1997
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Magnification of tributyl tin toxicity to oyster larvae by bioconcentration in biofilms of Shewanella colwelliana
Article Abstract:
A study was conducted on the influence of dissolved and bioconcentrated tributyl tin on oyster larvae. Shewanella colwelliana biofilm bioconcentration was observed to stem the metamorphosis and attachment of oyster larvae on bottom surfaces. Tributyl tin concentrations less than 5ng/ml exhibit no lasting effect on larval activity. Results have potential applications in environmental heavy metal toxicity studies.
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 1997
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