Use of monoclonal antibodies to demonstrate different sites with different functional characteristics in a bacterial lipase from Pseudomonas aeruginosa YS7
Article Abstract:
Monoclonal antibodies (MAbs) were used in this immunochemicalcharacterization of the extracellular lipase from Pseudomonas aeruginosa YS-7. Distribution of lipase binding properties among MAbs and their inhibitory effects on enzyme activity were found to be varied based on the results of screening and initial characterization. Binding of lipase to these MAbs showed the presence of structural or functional sites with specific effects on different enzyme functions. The binding sites used different binding mechanismswith the MAbs that affected the activity of the lipase. Lipase denaturation affected the binding site responsible for its hydrolytic activity but not the functional site involved in its hydrophobic attachment.
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 1992
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Direct and rapid analysis of the adhesion of bacteria to solid surfaces: interaction of fluorescently labeled Rhodococcus strain GIN-1 (NCIMB 40340) cells with titanium-rich particles
Article Abstract:
A fluorimetric assay using epifluorescence measurements helps in the study of adhesion of bacteria or microorganisms to any solid matrix. The assay makes use of labeling of the bacteria with fluorescamine that produces a yellow fluorescence on reaction with amino groups on the cell surface. Acetone used in the labeling reaction has no influence on the viability or adherence capacity of cells. The fluorescence labeling of Rhodococcus strain, GIN-1, verifies earlier findings that GIN-1 cells have higher affinities for TiO2, ZnO, and coal fly ash and lower affinities for other metal oxides.
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 1995
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Production, purification, and properties of a lipase from a bacterium (Pseudonomas aeruginosa YS-7) capable of growing in water-restricted environment
Article Abstract:
The extracellular lipase of Pseudonomas aeruginosa YS-7 was produced, purified and characterized. Significant increase in the enzyme production level was observed when different fatty acid esters such as triglycerides were used as carbon source. The crude enzyme was purified by hydrophobic interaction chomatography. Analysis showed that the lipase has 40 kDa and hydrolyzes different fatty acid esters. In addition, the rate of lypolysis was inversely related to the water content of the substrates.
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 1992
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