Use of morphology index histograms to quantify populations of the fungal pathogen Paracoccidioides brasiliensis
Article Abstract:
A method using morphology index (Mi) which was originally designed for the quantitative determination of Candida albicans populations was applied to Paracoccidioides brasiliensis. The method was intended to make the definition of cellular and molecular markers specific for cell development easy. It also aimed to determine the distribution of morphological types in different environmental conditions. The results of the study comparing wild and mutant strains of P. brasiliensis revealed that the Mi is inversely related to the content of both cell wall chitin and 1,3-alpha-glucan.
Publication Name: Microbiology
Subject: Biological sciences
ISSN: 1350-0872
Year: 1997
User Contributions:
Comment about this article or add new information about this topic:
Inhibition of Paracoccidioides brasiliensis by ajoene is associated with blockade of phosphatidylcholine biosynthesis
Article Abstract:
The effects of ajoene on the lipid composition of Paracoccidioides brasiliensis during the yeast-like (Y) and mycelial phase were analyzed to characterize the mechanisms of phosphatidylcholine (PC) synthesis. The exposure of Paracoccidioides brasiliensis cells to the platelet aggregation inhibitor reduced microbial PC composition. However, the antiproliferative effects of ajoene increased the levels of its precursor phosphatidylethanolamine and unsaturated fatty acids in the Y phase.
Publication Name: Microbiology
Subject: Biological sciences
ISSN: 1350-0872
Year: 1997
User Contributions:
Comment about this article or add new information about this topic:
Anionogenic groups and surface sialoglycoconjugate structures of yeast forms of the human pathogen Paracoccidioides brasiliensis
Article Abstract:
The surface anionogenic groups and sialoglycoconjugate structures of Paracoccidioides brasiliensis yeast forms were examined by cell microelectrophoresis, binding assays with lectins and viral particles, ultrastructural cytochemistry, enzymic digestion, and flow cytofluorimetry. Results showed that sialic acid residues are major anionogenic groups exposed on the yeast's surface and that P. brasiliensis yeast cell populations have heterogeneity.
Publication Name: Microbiology
Subject: Biological sciences
ISSN: 1350-0872
Year: 1998
User Contributions:
Comment about this article or add new information about this topic:
- Abstracts: Effect of low temperature on starvation-survival of the eel pathogen Vibrio vulnificus biotype 2. In vivo resuscitation, and virulence towards mice, of viable but nonculturable cells of Vibrio vulnificus
- Abstracts: Analysis of mechanisms regulating expression of the ver-1 gene involved in aflatoxin biosynthesis
- Abstracts: The ldh phylogeny for environmental isolates of Lactococcus lactis is consistent with rRNA genotypes but not with phenotypes
- Abstracts: Association of mercury resistance with antibiotic resistance in the gram-negative fecal bacteria of primates. Phylogeny of mercury resistance (mer) operons of gram-negative bacteria isolated from the fecal flora of primates
- Abstracts: A 10.3 kbp segment from nprB to argJ at the 102-degree region of the Bacillus subtilis chromosome. Sequencing of regions downstream of addA (98 degrees) and citG (289 degrees) in Bacillus subtilis