Use of quantum dot luminescent probes to achieve single-cell resolution of human oral bacteria in biofilms

Article Abstract:

The comparison of primary immunofluorescence by using quantum dots (QD)-antibody conjugates with primary immunofluorescence by using typical antibody fluorescence conjugates, such as Alexa Fluor conjugates is presented. The advances in the application of QD-based immunofluorescence for studying biofilms in vitro and in will help in defining bacterial community architecture and also in detecting the interactions between bacterial species in these communities.

Usage, Bacterial cell walls, Immunofluorescence, Fluorescent antibody technique, Report

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Molecular characterization of subject-specific oral microflora during initial colonization of enamel

Article Abstract:

The 16S rRNA gene sequencing and a retrievable enamel chip model were used to characterize the microflora present at 4 h and 8 h of plaque development in three individuals. Fluorescence in situ hybridization (FISH) was used to visualize the biofilm architecture of the mixed-species communities while conforming the dominance in situ of streptococci in initial dental plaque bacterial populations.

Development and progression, Fluorescence, Hybridization, Nucleotide sequencing

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Development of a multispecies oral bacterial community in a saliva-conditioned flow cell

Article Abstract:

The microbial communities amounting to more than 500 bacterial types are found in the human mouth. A saliva-conditioned flow cell was utilized with saliva as the only resource for nutrition in the example to check the formation of multispecies biofilm community and the results displayed that the biofilm communities were affected by the coaggregation in the planktonic phase.

Microbiology, Saliva

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