Use of semiconductor quantum dots for photostable immunofluorescence labeling of Cryptosporidium parvum
Article Abstract:
Studies done to develop a specific, sensitive and photostable detection method for C.parvum oocysts by using luminescence QD-streptavidin together with biotinylated anti-Cryptosporidium sp.monoclonal antibody (MAb) are reported. It is demonstrated that the QD-streptavidin with biotinylated MAb was able to provide a sensitive technique for detecting C.parvum oocysts with the advantage of having a high photobleaching threshold.
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 2004
User Contributions:
Comment about this article or add new information about this topic:
An alternative efficient procedure for purification of the obligate intracellular fish bacterial pathogen Piscirickettsia salmonis
Article Abstract:
Research describes the isolation of pure Piscirickettsia salmonis using a density gradient centrifugation procedure that uses iodixanol as a separation medium. Iodixanol is a high density and low osmolarity substance. Data indicate that the low osmolarity of iodixanol facilitates better resolution of the bacteria from the membranous structires of the host cell.
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 2003
User Contributions:
Comment about this article or add new information about this topic:
Effect of particles on the recovery of Cryptosporidium oocysts from source water samples of various turbidities
Article Abstract:
Research indicates that suspended particles in reservoir water can be helpful in the recovery of oocysts using filtration step in the recovery methodology. Data indicate that particle size and concentration improve oocyte recovery, as such adding particles to a water matrix in the U.S. Environmental Protection Agency developed method 1622 is reccommended.
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 2003
User Contributions:
Comment about this article or add new information about this topic:
- Abstracts: Method for detection and enumeration of Cryptosporidium parvum oocysts in feces, manure and soils. Rapid extraction of DNA from Escherichia coli and Cryptosporidium parvum for use in PCR
- Abstracts: Induction of folds or sutures on the walls of Cryptosporidium parvum oocysts and their importance as a diagnostic feature
- Abstracts: Evaluation of PCR, nested PCR, and fluorescent antibodies for detection of Giardia and Cryptosporidium species in wastewater
- Abstracts: Use of autoradiography to assess viability of Helicobacter pylori in water. Colonization of broiler chickens by waterborne Campylobacter jejuni
- Abstracts: The earthworm gut: an ideal habitat for ingested nitrogen oxide-producing microorganisms. N(sub)2O-producing microorganisms in the gut of the earthwork Aporrectodea caliginosa are indicative of ingested soil bacteria