Induction of folds or sutures on the walls of Cryptosporidium parvum oocysts and their importance as a diagnostic feature
Article Abstract:
The proportion of oocysts of Cryptosporidium parvum, having a fold on oocyst walls when incubated with either flucorescent monoclonal antibody or a surface-reactive fluorescent dye, was increased by incubating suspensions of oocysts with dimethyl sulfoxide, sucrose or Hank's balanced salt solution. Further incubation of sucrose-incubated oocysts with water indicated that this was reversible. Oocysts demonstrating this fold after incubation in dimethyl sulfoxide were of the same viability as control oocysts and followed the same excystation dynamics.
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 1993
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Detection of UV-induced thymine dimers in individual Cryptosporidium parvum and Cryptosporidium hominis Oocysts by immunofluorescence microscopy
Article Abstract:
The effect of UV light on Cryptosporidium parvum and Cryptosporidium hominis oocysts in vitro was investigated by exposing the intact oocysts to 4-, 10-, 20-, and 40-mJ [cm.sup.-2] doses of UV irradiation. The results suggest that the immunofluorescence assay is suitable for detecting DNA damage in Cryptosporidium parvum and Cryptosporidium hominis oocysts induced following exposure to UV light.
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 2007
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- Abstracts: Method for detection and enumeration of Cryptosporidium parvum oocysts in feces, manure and soils. Rapid extraction of DNA from Escherichia coli and Cryptosporidium parvum for use in PCR
- Abstracts: Preparation of refractile spores of Clostridium thermosaccharolyticum involves a solventogenic phase. Isolation and characterization of an extracellular glycosylated protein complex from Clostridium thermosaccharolyticum with pectin methylesterase and polygalacturonate hydrolase activity
- Abstracts: Viability of Cryptosporidium parvum oocysts: correlation of in vitro excystation with inclusion or exclusion of fluorogenic vital dyes