Vaccinia NPH-I, a DExH-box ATPase, is the energy coupling factor for mRNA transcription termination

Article Abstract:

A termination protein in vaccinia virus that catalyzes ATP hydrolysis and is closely associated with a polymerase-elongation complex has been identified as nucleoside triphosphate phosphohydrolase-I (NPH-I). It is a virus-encoded DNA-dependent ATPase of the DExH-box family. NPH-I has roles in transcription, one with VTF/CE to catalyze release of UUUUUNU-containing nascent RNA from the elongation complex and one alone as a polymerase elongation factor to make readthrough of intrinsic pause sites easier. Vaccinia NPH-I is the energy-coupling factor for eukaryotic transcription termination for mRNA.

Methods, Molecular biology, Nucleosides, Nucleotidases, Vaccinia

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Regulation of the fission yeast transcription factor Pap1 by oxidative stress: requirement for the nuclear export factor Crm1 (exportin) and the stress-activated MAP kinase Sty1/Spc1

Article Abstract:

Regulation of the Pap1 transcription factor by Sty1 controls cellular response to oxidative stress as well as to cytotoxic agents in fission yeast. Expression of genes involved in oxidative stress response and in multidrug resistance is determined by both Sty1 and Pap1. Oxidative stress causes relocalization of the Pap1 protein to the nucleus from the cytoplasm, a process which requires Sty1 kinase. Relocalization occurs through regulated protein export involving Crm1 (exportin) nuclear export factor and a gene that encodes an Ran nucleotide exchange factor.

Genetic aspects, Oxidation, Physiological, Physiological oxidation, Stress (Physiology), Yeast, Yeast (Food product), Protein kinases

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The competence transcription factor of Bacillus subtilis recognizes short A/T-rich sequences arranged in a unique flexible pattern along the DNA helix

Article Abstract:

ComK is the competence transcription factor in Bacillus subtilis. Short A/T-rich sequences in a specific pattern are recognized by ComK. ComK probably operates as a tetramer. Three categories of promoters regulated by ComK have been identified. The research suggests that binding of ComK occurs at the minor groove on one face of the DNA helix.

Bacillus subtilis

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