Viability of Cryptosporidium parvum oocysts: correlation of in vitro excystation with inclusion or exclusion of fluorogenic vital dyes

Article Abstract:

Propidiumiodide (PI) and 4',6-diamidino-2-phenylindole (DAPI) are fluorogenic vital dyeswhich were previously shown to be promising candidates for assessing viability of protozoan cysts and oocysts. The inclusion or exclusion of these dyes were compared to in vitro excystation of Cryptosporidium parvum oocysts. The resultsshowed that oocysts which are permeable to DAPI but not to PI will excyst usinga standard excystation protocol. Simultaneous staining using DAPI and PI is a useful protocol to determine the viability of C. parvum oocysts.

Methods, Stains and staining (Microscopy), Stains (Microscopy), Pathogenic microorganisms, Protozoa, Pathogenic

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Survival of Cryptosporidium parvum oocysts under various environmental pressures

Article Abstract:

The effects of freezing, desiccation, water treatment, fecal environment and various water types on the survival of various isolates of Cryptosporidium parvum oocysts were investigated. The results showed that some oocysts could survive temperatures of down to -22 degrees celsius. Desiccation was invariably lethal, while water treatment processes did not affect the survival of oocysts under correct pH conditions. Oocysts survived in all water types, while a fecal environment resulted in an enhanced impermeability to small molecules.

Physiological aspects, Environmental aspects, Protozoa

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Viability of Cryptosporidium parvum oocysts: assessment by the dye permeability assay

Article Abstract:

Research made by Michael B. Jenkins et al on the permeability and survival of Cryptosporidium parvum provided interesting pertinent data on the topic. However, there are certain arguable points that they raised on the viability of oocysts and their use of recommended pretreatment solely on oocysts to be excysted. Further research on simultaneous viability assessment and detection is recommended to be treated with caution for environmental monitoring.

Criticism and interpretation, Permeability, Jenkins Michael B., Anguish, Lynne J., Ghiorse, William C., Bowman, Dwight D., Walker, Mark J.

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