Transformation of chlorinated benzenes and toluenes by Ralstonia sp. strain PS12 tecA (tetrachlorobenzene dioxygenase) and tecB (chlorobenzene dihydrodiol dehydrogenase) gene products
Article Abstract:
Research has been conducted on the tecB gene located downstream of tecA and encoding tetrachlorobenzene dioxygenase in Ralstonia sp. strain PS12. The results of the tecB gene cloning into the Escherichia coli DH5alpha together with the tecA gene are described.
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 2001
User Contributions:
Comment about this article or add new information about this topic:
Rational engineering of the regioselectivity of TecA tetrachlorobenzene dioxygenase for the transformation of chlorinated toluenes
Article Abstract:
Research has been conducted on Ralstonia sp. PS12 tetrachlorobenzene dioxygenase. The authors have investigated this dioxygenase which is involved in chlorinated toluene biodegradation, and report the results.
Publication Name: Microbiology
Subject: Biological sciences
ISSN: 1350-0872
Year: 2003
User Contributions:
Comment about this article or add new information about this topic:
cis-Chlorobenzene dihydrodiol dehydrogenase (TcbB) from Pseudomonas sp. strain P51, expressed in Escherichia coli DH5alpha (pTCB149), catalyzes enantioselective dehydrogenase reactions
Article Abstract:
Pseudomonas sp. strain P51 cis-chlorobenzene dihydrodiol dehydrogenase (CDD)(TcbB) expressed in Escherichia coli DH5alpha (pTCB149) has been found to catalyze enantioselective dehydrogenase reactions. The study was carried out with the object of investigating the potential of CDD for enantiomeric resolution of chiral dihydrodiols. CDD preferentially oxidized the para-halogenated cis-toluene dihydrodiol enantiomers preferentially formed by chlorobenzene dioxygenase (CDO).
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 1999
User Contributions:
Comment about this article or add new information about this topic:
- Abstracts: Thermostable NADP(super +)-dependent medium-chain alcohol dehydrogenase from Acinetobacter sp. strain M-1: purification and characterization and gene expression in Escherichia coli
- Abstracts: Characterization of 4-hydroxyphenylacetate 3-hydroxylase (HpaB) of Escherichia coli as a reduced flavin adenine dinucleotide-utilizing monooxygenase
- Abstracts: Purification and characterization of the recombinant Thermus sp. strain T2 alpha-galactosidase expressed in Escherichia coli
- Abstracts: Controlled autolysis and enzyme release in a recombinant lactococcal strain expressing the metalloendopeptidase enterolysin A
- Abstracts: Metabolic engineering of mannitol production in Lactococcus lactis: Influence of overexpression of mannitol 1-phosphate dehydrogenase in different genetic backgrounds