Exploitation of plasmid pMRC01 to direct transfer of mobilizable plasmids into commercial lactococcal starter strains
Article Abstract:
Researchers have discovered a genetic sequence on the lactococcal plasmid pMRC01 that resembles an origin of transfer (oriT). This oriT could be used to in a vector to genetically modify lactococcal starter strains used in the food industry.
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 2001
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Production of enterolysin A by a raw milk enterococcal isolate exhibiting multiple virulence factors
Article Abstract:
Research reveals that the DPC5280 strain of Enterococcus faecalis, isolated from Irish raw milk, displays antimicrobial activity against gram-positive bacteria. Data indicate that the inhibition is due to a cytolysin and an antimicrobial protein. The protein exhibits cell-wall-degrading activity and is similar to the cell-wall degrading enzyme, enterolysin A.
Publication Name: Microbiology
Subject: Biological sciences
ISSN: 1350-0872
Year: 2003
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Controlled autolysis and enzyme release in a recombinant lactococcal strain expressing the metalloendopeptidase enterolysin A
Article Abstract:
The possibility of exploiting lytic enzyme enterolysin A(EntL) in applications involving lactococcal starter cultures is investigated. A recombinant lactococcal strain is generated which expresses EntL under the control of the nisin-inducible promoter and consequently allows nisin-induced autolysis.
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 2004
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