p53 protein exhibits 3'-to-5' exonuclease activity

Article Abstract:

The p53 protein shows exonuclease activity and degrades DNA with a 3'-to-5' polarity in the presence of Mg2+. The exonuclease activity is specific for the purified wild-type protein as p53 mutants lack the activity. The activity copurifies with p53 during the preparation of the wild-type protein but fails to copurify with mutant p53. The enzyme activity can be reconstituted from urea-denatured and SDS gel-purified p53 and mapped to the core region of the p53 molecule.

Usage, Physiological aspects, Gel electrophoresis, Proteins, Mutation (Biology), Mutation, Protein denaturation, Exonucleases

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The menin tumor suppressor protein is an essential oncogenic cofactor for MLL-associated leukemogenesis

Article Abstract:

Oncogenic MLL fusion proteins retained an ability to stably associate with menin through a high-affinity, amino-terminal, conserved binding motif and this interaction was required for the initiation of MLL-mediated leukemogenesis. Acute genetic ablation of menin reversed aberrant Hox gene expression mediated by MLL-menin promoter-associated complexes, and oncogenic properties of MLL-transformed leukemic blasts.

Protein binding, Genetic research

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A novel human p53 isoform is an essential element of the ATR-intra-S phase checkpoint

Article Abstract:

Evidence is provided for the existence of an alternatively spliced p53 isoform (delta p53) that exerts its transcriptional activity independent from p53. Cell cycle studies showed that delta-p53 displays its differential transcriptional activity only in damaged ATR-intra-S phase cells.

Cell cycle

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