rRNA stability in heat-killed and UV-irradiated enterotoxigenic Staphylococcus aureus and Escherichia coli O157:H7
Article Abstract:
The stability of 16S rRNA in Escherichia coli O157:H7 and Staphylococcus aureus was investigated to determine whether the molecule can be used as an indicator of cell viability in nucleic acid-abased detection assays. The stability of the molecule was analyzed using Northern hybridization and on the basis of its ability to support reverse transcription-polymerase chain reaction amplification after cells were killed by heat or ultraviolet (UV) irradiation. Results show that the rRNA is suitable for monitoring bacterial viability under extreme heat conditions, but not after UV irradiation.
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 1998
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Immobilization with metal hydroxides as a means to concentrate food-borne bacteria for detection by cultural and molecular methods
Article Abstract:
Metal hydroxides and their immobilization as a way to concentrate food-borne bacteria for detecting using molecular and cultural methods are discussed. This application was studied by working with concentrations of bacterial cells from dairy foods using nonfat dry milk as a model and Listeria monocytogenes and Salmonella enterica serovar Enteritidis. Zirconium hydroxide was used. Bacterial immobilization is fast, easy, and not expensive and may be useful in concentrating a wide range of food-borne bacteria before detection using alternative and conventional methods.
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 2000
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Activities of garlic oil, garlic powder, and their diallyl constituents against Helicobacter pylori
Article Abstract:
Garlic powder (GP), garlic oil (GO), and their diallyl constituents and activity against Helicobacter pylori have been studied to assess the in vivo anti- H. pylori potential of each. It was found that GP, GO and their diallyl sulfur components have substantial in vitro anti-H. pylori effects. A potential for in vivo clinical use for infections with H. pylori is suggested by results.
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 2000
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