Intein-based biosynthetic incorporation of unlabeled protein tags into isotopically labeled proteins for NMR studies

Article Abstract:

The segmental isotopic labeling of proteins is conveniently achieved in Escherichia coli using intein-based protein ligation is demonstrated. Using the in vivo approach, unlabeled protein tags can be incorporated into isotopically labeled target proteins to improve protein stability and solubility for study by solution NMR spectroscopy.

Usage, Observations, Nuclear magnetic resonance spectroscopy, Protein binding

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Semisynthetic production of unnatural L-alpha-amino acids by metabolic engineering of the cysteine-biosynthetic pathway

Article Abstract:

A fermentation based approach for biosynthesis of unnatural L-alpha-amino acids after re-engineering the cysteine-biosyntheric pathway in Escherichia coli is presented. The conclusion states that o-acetyl-L-serine extruded from the cells by way of the o-acetylserine efflux protein was amenable to further biotransfornmations.

Amino acids, Metabolic therapies

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Cold-shock induced high-yield protein production in Escherichia coli

Article Abstract:

The excessive expression of proteins in Escherichia coli at low temperature enhances their constancy and solubility. However, the distinct characteristics of cspA gene are needed to create a range of expression of cloned genes with the induction of cold-shock.

Analysis, Causes of, Influence, Protein engineering, Cryobiology

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