Generic expansion of the substrate spectrum of a DNA polymerase by directed evolution
Article Abstract:
A strategy to expand the substrate range of DNA polymerases that recognize their substrates with exceptionally high specificity, restricting the use of unnatural nucleotides and the applications they enable. Mutants of Taq DNA polymerase that not only extended mismatches, but had acquired a generic ability to process a diverse range of noncanonical substrates is obtained while maintaining high catalytic turnover, processivity and fidelity.
Publication Name: Nature Biotechnology
Subject: Business
ISSN: 1087-0156
Year: 2004
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Molecular breeding of polymerases for amplification of ancient DNA
Article Abstract:
A strategy is described for the recovery of genetic information from damaged DNA. The results have shown that engineered polymerases can expand the recovery of genetic information from Pleistocene specimens and might benefit genetic analysis in paleontology, archeology and forensic medicine.
Publication Name: Nature Biotechnology
Subject: Business
ISSN: 1087-0156
Year: 2007
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Comment about this article or add new information about this topic:
Molecular breeding of polymerases for amplification of ancient DNA
Article Abstract:
A strategy is described for the recovery of genetic information from damaged DNA. The results have shown that engineered polymerases can expand the recovery of genetic information from Pleistocene specimens and might benefit genetic analysis in paleontology, archeology and forensic medicine.
Publication Name: Nature Biotechnology
Subject: Business
ISSN: 1087-0156
Year: 2007
User Contributions:
Comment about this article or add new information about this topic:
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