Quantitative proteomic assessment of very early cellular signaling events

Article Abstract:

An automated, continuous quench-flow system allowing quantitative proteomic assessment of very early cellular signaling events (qPACE) with a time resolution of 1s was developed. This technique helped in determining the fact that autophosphorylation of the epidermal growth factor receptor occurs within 1 s after ligand simulation and is followed by phosphorylation of the downstream signaling intermediates Src homologous and proteins.

Science & research, Research, Phosphorylation, Protein research, Epidermal growth factor receptors

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A method for the comprehensive proteomic analysis of membrane proteins

Article Abstract:

An analysis of membrane and soluble proteins utilizing a high pH and proteinase K method is discussed.

Cover Story

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Membrane proteins ride shotgun

Article Abstract:

The biological functions of membrane proteins are discussed.

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