Reversal of mouse hepatic failure using an implanted liver-assist device containing ES cell-derived hepatocytes
Article Abstract:
Mouse embryonic stem (ES) cells are differentiated into hepatocytes by coculture with a combination of human liver nonparenchymal cell lines and fibroblast growth factor-2, human activin A and hepatocyte growth factor to generate an alternative source of cells for bioartificial liver support. This kind of differentiation provides a foundation for differentiation of functional hepatocytes from human ES cells and a practical design for a clinically useful liver-assist device.
Publication Name: Nature Biotechnology
Subject: Business
ISSN: 1087-0156
Year: 2006
User Contributions:
Comment about this article or add new information about this topic:
A human beta-cell line for transplantation therapy to control type 1 diabetes
Article Abstract:
A reversibly immortalized human beta-cell clone (NAKT-15) is established by transfection of primary human beta-cells with a retroviral vector containing simian virus 40 large T-antigen (SV40T) and human telomerase reverse transcriptase (hTERT) cDNA flanked by paired IoxP recombination targets. Transplantation of NAKT-15 cells into streptozotocin-induced diabetic severe combined immunodeficiency mice resulted in perfect control of blood glucose within 2 weeks.
Publication Name: Nature Biotechnology
Subject: Business
ISSN: 1087-0156
Year: 2005
User Contributions:
Comment about this article or add new information about this topic:
Islets for all?
Article Abstract:
A report on the application of a reversible immortalization strategy for expanding human islets cells with retention of function is presented. Immortalized cell lines are generated by transformation of human islet cells with retroviral vectors expressing SV40 large T-antigen and human telomerase reverse transcriptase (TERT), with other of these genes flanked by loxP sites for later Cre recombinase-based excision.
Publication Name: Nature Biotechnology
Subject: Business
ISSN: 1087-0156
Year: 2005
User Contributions:
Comment about this article or add new information about this topic:
- Abstracts: Ex vivo generation of fully mature human red blood cells from hematopoietic stem cells. Stem cell-derived erythroid cells mediate long-term systemic protein delivery
- Abstracts: Fundamental determinants of national equity market returns: a perspective on conditional asset pricing. Risk management implications of time-inconsistency: Model updating and recalibration of no-arbitrage model
- Abstracts: Improving the nutritional value of Golden Rice through increased pro-vitamin A contentPaine. part 2 Steroid regulation improves crop yield
- Abstracts: Linear temporal characteristics of heart interbeat interval as an index of the pilot's perceived risk. Complete recovery time after exhaustion in high-intensity work
- Abstracts: Gap Inc. sees supplier ownership of compliance with workplace standards as an essential element of socially responsible sourcing