Fluorescence correlation spectroscopy analysis of diffusion in a laser gradient field: A numerical approach
Article Abstract:
Biased fluorescence correlation spectroscopy (FCS) curves can be calculated numerically and fitted to the experimental data to retrieve the unbiased particle number, diffusion time, and polarizability of the fluorescent particles as well as the strength of the gradient field. It is proven using simulated FCS data that the discrepancy caused by the spherical symmetry approximation is independent of the gradient field strength and therefore it can be eliminated.
Publication Name: Journal of Physical Chemistry B
Subject: Chemicals, plastics and rubber industries
ISSN: 1520-6106
Year: 2005
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Extended photon counting histogram and fluorescence intensity distribution analysis approaches for optically biased photon counting statistics
Article Abstract:
The theories of the photon counting histogram (PCH) and fluorescence intensity distribution analysis (FIDA) approaches are extended to analyze quantitatively the photon counting spectra in a laser gradient field. The efforts provide the ability to evaluate the interaction between the optical field and the particles as well as to measure the actual local concentration and the particle brightness from biased detections.
Publication Name: Journal of Physical Chemistry B
Subject: Chemicals, plastics and rubber industries
ISSN: 1520-6106
Year: 2006
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A comparison between photon counting histogram and fluorescence intensity distribution analysis
Article Abstract:
Photon counting histogram (PCH) and fluorescence intensity distribution analysis (FIDA), the two methods, are examined in details for a direct comparison. It is demonstrated that FIDA and PCH are equivalent not only in the statistical method and the theoretical formulations but also in the manner of modeling experimental point spread functions (PSFs).
Publication Name: Journal of Physical Chemistry B
Subject: Chemicals, plastics and rubber industries
ISSN: 1520-6106
Year: 2006
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