Stepping statistics of single HIV-1 reverse transcriptase molecules during DNA polymerization

Article Abstract:

Results from a new kind of single-molecule kinetic measurement on the human immunodeficiency virus (HIV) reverse transcriptase are reported. The intrinsic processive DNA-dependent polymerization of HIV reverse transcriptase (RT) is approximately Poissionian, wherein each nucleotide is added sequentially, with a rate of about 100 bases per second at 21 degree Celsius.

HIV (Viruses), HIV, DNA polymerases

---

Thermodynamic properties of single ion channel formation: Gramicidin

Article Abstract:

Single molecule fluorescence imaging is used to unequivocally differentiate between rhodamine-6G labeled gramicidin monomeric subunits and channel-forming dimers. The findings allow complete characterization of the thermodynamic properties of dimer formation, 2G(sub 1)<-->G(sub 2) necessary for channel function.

All Other Basic Organic Chemical Manufacturing, Vinyl Chloride Monomer, Thermodynamics, Monomers, Gramicidins

---

The excision mechanism in reverse transcriptase: pyrophosphate leaving and fingers opening are uncoupled events with the analogues AZT and d4T

Article Abstract:

Molecular dynamics simulations are used for examining the complexes of HIV-1 reverse transcriptase (RT) with the substrate and the antiretrovirals AZT and d4t. Results suggest that the finger residues K65 and R72 form hydrogen bonds with the substituted nucleotides.

Antiviral agents, Hydrogen bonding, Hydrogen bonds, Chemical properties, Antiretroviral agents

Similar abstracts:

• Abstracts: Theoretical studies of manganese and iron superoxide dismutases: Superoxide binding and superoxide oxidation. The Ti(sub 8)C(sub 12) metcar: A new model catalyst for hydrodesulfurization
• Abstracts: Structure, energetics, and dynamics of water adsorbed on the muscovite (001) surface: A molecular dynamics simulation
• Abstracts: Influence of thiol capping on the exciton luminescence and decay kinetics of CdTe and CdSe quantum dots. Luminescence lifetime distributions analysis in heterogeneous systems by the use of Excel's Solver
• Abstracts: Bending and radial deformation of lipid tubules on self-assembled thiol monolayers. Nondissociative chemisorption of short chain alkanethiols on Au(111)
• Abstracts: In situ studies of phase transitions in thin discotic films. Interfacial study of cubic boron nitride films deposited on diamond

This website is not affiliated with document authors or copyright owners. This page is provided for informational purposes only. Unintentional errors are possible.