Stepping statistics of single HIV-1 reverse transcriptase molecules during DNA polymerization
Article Abstract:
Results from a new kind of single-molecule kinetic measurement on the human immunodeficiency virus (HIV) reverse transcriptase are reported. The intrinsic processive DNA-dependent polymerization of HIV reverse transcriptase (RT) is approximately Poissionian, wherein each nucleotide is added sequentially, with a rate of about 100 bases per second at 21 degree Celsius.
Publication Name: Journal of Physical Chemistry B
Subject: Chemicals, plastics and rubber industries
ISSN: 1520-6106
Year: 2005
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Thermodynamic properties of single ion channel formation: Gramicidin
Article Abstract:
Single molecule fluorescence imaging is used to unequivocally differentiate between rhodamine-6G labeled gramicidin monomeric subunits and channel-forming dimers. The findings allow complete characterization of the thermodynamic properties of dimer formation, 2G(sub 1)<-->G(sub 2) necessary for channel function.
Publication Name: Journal of Physical Chemistry B
Subject: Chemicals, plastics and rubber industries
ISSN: 1520-6106
Year: 2004
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The excision mechanism in reverse transcriptase: pyrophosphate leaving and fingers opening are uncoupled events with the analogues AZT and d4T
Article Abstract:
Molecular dynamics simulations are used for examining the complexes of HIV-1 reverse transcriptase (RT) with the substrate and the antiretrovirals AZT and d4t. Results suggest that the finger residues K65 and R72 form hydrogen bonds with the substituted nucleotides.
Publication Name: Journal of Physical Chemistry B
Subject: Chemicals, plastics and rubber industries
ISSN: 1520-6106
Year: 2007
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