Time-resolved CINDP study of non-native states of bovine and human alpha-lactalbumins
Article Abstract:
The reaction mechanism and details of the formation of CIDNP (chemically induced dynamic nuclear polarization) in the photoreactions of the aromatic dye 2,2'-dipyridyl with non-native states of bovine and human alpha-lactalbumins in aqueous solution are studied using the time-resolved CIDNP technique. Relative intensities of the signals in the geminate CIDNP spectra can be used to give information on the accessibility and reactivity of amino acid residues toward the excited dye molecules.
Publication Name: Journal of Physical Chemistry B
Subject: Chemicals, plastics and rubber industries
ISSN: 1520-6106
Year: 2005
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Intramolecular electron transfer in lysozyme studied by time-resolved chemically induced dynamic nuclear polarization
Article Abstract:
The kinetics of the chemically induced dynamic nuclear polarization (CIDNP) produced in reactions of hen lysozyme with photosensitizers was studied for the native state of protein. The detailed analysis of the CIDNP time dependence on a microsecond time scale revealed that in both denatured states, intramolecular electron transfer from a tyrosine residue to the cation radical of tryptophan residue is highly efficient and plays a decisive role in the evolution of the nuclear polarization.
Publication Name: Journal of Physical Chemistry B
Subject: Chemicals, plastics and rubber industries
ISSN: 1520-6106
Year: 2005
User Contributions:
Comment about this article or add new information about this topic:
Time-resolved CIDNP study of native-state bovine and human alpha-lactalbumins
Article Abstract:
The reaction mechanism and details of the formation of CIDNP in the photoreactions of two aromatic dyes with bovine and human alpha-lactalbumins in aqueous solution is examined with the help of time-resolved CIDNP technique. The correlation times for the side chain motion as determined from the T1 of the radicals are correlated with the accessibility of the side chains in the intact protein.
Publication Name: Journal of Physical Chemistry B
Subject: Chemicals, plastics and rubber industries
ISSN: 1520-6106
Year: 2004
User Contributions:
Comment about this article or add new information about this topic:
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