Cellular ascorbate depletion in healthy men

Article Abstract:

Cellular ascorbate depletion studies were made on eight healthy men aged 25 to 43 to determine the effect of vitamin C deficiency on sperm fertility. The men were fed diets with graded ascorbic acid amounts for 13 weeks. Short-term ascorbic acid deficiency appeared to affect semen agglutination and motility but not fertility-associated properties such as volume, pH or liquefaction time. Moderate ascorbic acid supplementation did not improve fertility. Findings also suggest that plasma ascorbic acid level reflects leucocyte ascorbic acid content more accurately than buccal cell ascorbic acid levels.

Health aspects, Fertility, Human, Human fertility, Vitamin C deficiency

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Plasma carotenoid levels in human subjects fed a low carotenoid diet

Article Abstract:

The influence of dietary constraints on plasma carotenoid levels was examined in 12 healthy male humans. The subjects were given low carotenoid diet and plasma carotenoid concentrations were monitored for 13 weeks. Results showed a significant reduction in plasma carotenoid levels within the first two weeks. This indicates a considerable and almost instantaneous effect of carotenoid deficiency on serum carotenoids. Further, this suggests the necessity of regular intake of carotenoid-rich foods to maintain adequate carotenoid concentrations in the plasma.

Physiological aspects, Carotenoids, Nutrition

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Collagen synthesis in human skin fibroblasts is stimulated by a stable form of ascorbate, 2-O-alpha-D-glucopyranosyl-L-ascorbic acid

Article Abstract:

2-O-alpha-D-glucopyranosyl-L-ascorbic acid (AA-2G) is a novel L-ascorbic acid glucoside which is highly stable in various aqueous solutions in a wide range of pH and reducing condition. An investigation was conducted to determine the effectiveness of AA-2G in maintaining cell cultures. The results showed that AA-2G is very stable in culture media and can stimulate collagen synthesis in cultured skin fibroblasts. Therefore, AA-2G will be a useful supplement in skin fibroblast cultures as well as in other multicomponent liquid products.

Culture media (Biology), Culture media, Cell culture, Tissue culture, Fibroblasts

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