Secretory patterns of biotin in human milk
Article Abstract:
The effect of sampling time, breast side, breast emptying and postpartum interval on the biotin concentration in human milk is evaluated to determine a suitable sampling procedure that may accurately estimate biotin levels in milk. Results show considerable individual variations on the effect of different factors on milk biotin levels. Consequently, biotin in human milk exhibit pronounced fluctuations. Frequent sampling from both breasts is recommended in order to make a reasonable assessment of biotin levels in milk.
Publication Name: The Journal of Nutrition
Subject: Food/cooking/nutrition
ISSN: 0022-3166
Year: 1992
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Biotin in human milk: methods, location and chemical form
Article Abstract:
A technique analyzing biotin levels in human milk is presented. The assay makes use of avidin in the analysis of human milk composition. It is shown that receptacle composition do not affect biotin content of milk. Different temperatures have varying influence on milk's biotin content. Compartmentalization of biotin distribution is also assessed. It is concluded that the assay can detect nearly all of the free biotin in a milk sample.
Publication Name: The Journal of Nutrition
Subject: Food/cooking/nutrition
ISSN: 0022-3166
Year: 1992
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Biotin deficiency in rats: disturbances of leucine metabolism are detectable early
Article Abstract:
A study was done on the detection of disturbances of leucine metabolism observed during the course of biotin deficiency. 3-hydroxyisovaleric acid which is the shunted product of the biotin-dependent enzyme 3-methylcrotonyl-CoA was accurately measured in rats deficient in biotin for 16 days. These findings suggest that biotin deficiency can be detected early in rats as long as analytical errors are minimized.
Publication Name: The Journal of Nutrition
Subject: Food/cooking/nutrition
ISSN: 0022-3166
Year: 1992
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