Detection of HIV vaccine-induced cell-mediated immunity in HIV-seronegative clinical trial participants using an optimized and validated enzyme-linked immunospot assay
Article Abstract:
The interferon-[gamma] (IFN[gamma]) enzyme-linked immunospot (ELISPOT) assay is used to detect a broad range of HIV-specific ELISPOT responses to peptide pools of overlapping 22mers, 15mers, or 9mers in study volunteers receiving DNA- or adenovirus vector-based HIV vaccines and in HIV-seropositive donors. The results have shown that the validated ELISPOT assay using 15mer peptide pools and the positivity criteria is a sensitive and specific assay for the detection of HIV vaccine-induced cell-mediated immunity.
Publication Name: Journal of Acquired Immune Deficiency Syndromes (1999)
Subject: Health
ISSN: 1525-4135
Year: 2007
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Comment about this article or add new information about this topic:
Detection of HIV vaccine-induced cell-mediated immunity in HIV-seronegative clinical trial participants using an optimized and validated enzyme-linked immunospot assay
Article Abstract:
The interferon-[gamma] (IFN[gamma]) enzyme-linked immunospot (ELISPOT) assay is used to detect a broad range of HIV-specific ELISPOT responses to peptide pools of overlapping 22mers, 15mers, or 9mers in study volunteers receiving DNA- or adenovirus vector-based HIV vaccines and in HIV-seropositive donors. The results have shown that the validated ELISPOT assay using 15mer peptide pools and the positivity criteria is a sensitive and specific assay for the detection of HIV vaccine-induced cell-mediated immunity.
Publication Name: Journal of Acquired Immune Deficiency Syndromes (1999)
Subject: Health
ISSN: 1525-4135
Year: 2007
User Contributions:
Comment about this article or add new information about this topic:
Cross-clade reactivity of HIV-1-specific T-cell responses in HIV-1-infected individuals from Botswana and Cameroon
Article Abstract:
Anti-HIV-1 T-cell immune responses among 363 unvaccinated adults infected with diverse HIV-1 clades is compared. It is revealed that the response rates to clade B Gag and/or clade B Nef in Botswana, with 95 percent and Cameroon, with 98 percent are similar.
Publication Name: Journal of Acquired Immune Deficiency Syndromes (1999)
Subject: Health
ISSN: 1525-4135
Year: 2006
User Contributions:
Comment about this article or add new information about this topic:
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