A whole blood alternative to traditional methods for CD4+ T lymphocyte determination

Article Abstract:

An innovative method for determining T cell subtypes may be effective in developing countries that can not perform flow cytometry. The method involves coating small microspheres called CytoSpheres with monoclonal antibodies against CD4 and CD8 T cells. Then the microspheres are mixed with whole blood to allow the microspheres to bind to T cells. The blood sample is then treated with chemicals to lyse the blood cells and the sample can then be examined under a regular microscope in conjunction with a hemocytometer.

Usage, Measurement, T cells, Blood, Monoclonal antibodies, Blood chemical analysis

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Ritonavir combination therapy restores intestinal function in children with advanced HIV disease

Article Abstract:

The authors tested a combination of drugs, including ritonavir, in HIV-positive children whose intestines were losing the ability to absorb nutrition. The combination restored intestinal function very quickly and the children began gaining weight and increasing their CD4 count.

Evaluation, Physiological aspects, Ritonavir, HIV infection in children, Pediatric HIV infections, Drug therapy, Combination, Combination drug therapy, Intestinal absorption

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